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Single B Cell Antibody Discovery Service

As the biotechnology industry grows, the need for dependable and efficient antibody discovery services becomes more critical. Single B-cell screening is a cutting-edge technology that has emerged as an effective tool for discovering novel antibodies with high specificity and affinity. We are at the forefront of this innovation at Creative Biolabs, providing comprehensive single B-cell antibody discovery services to support a wide range of research and clinical applications.


The single B-cell antibody discovery process typically involves several steps, including cell isolation, single-cell sorting, antibody gene amplification, and sequence analysis. At Creative Biolabs, we have developed a streamlined workflow that ensures high-quality results at every stage of the process.

First, we isolate B-cells from the immunized animal or human donor using flow cytometry. We then use our proprietary Single B technology to sort individual B-cells and deposit them into individual wells of a 96-well plate. This ensures the isolation of single, viable B-cells that can be used for downstream analysis.

Next, we use our advanced single-cell amplification technology to amplify the antibody genes from each B-cell. This process allows for the efficient recovery of full-length variable heavy and light chain sequences. Once the sequences are obtained, we use bioinformatics tools to analyze and identify high-affinity antibody candidates.

Finally, we produce recombinant antibodies using our state-of-the-art expression systems and conduct extensive characterization to validate their efficacy and specificity.

Workflow of our single B cell antibody discovery service.Fig.1 Workflow of our single B cell antibody discovery service. (Creative Biolabs)

Our Advantages

Creative Biolabs offers a number of advantages with its single B cell antibody discovery service, including:

  • High specificity and affinity
    The use of single B cell screening and analysis technologies allows for the identification and isolation of B cells with high specificity and affinity for a particular antigen. This ensures that the resulting monoclonal antibodies have the desired properties for various applications.
  • Customization
    The single B cell antibody discovery service provided by Creative Biolabs is highly customizable, allowing for the selection of various immunization protocols, antigen design, and antibody purification and characterization options. This ensures that the resulting monoclonal antibodies meet the specific needs of each customer.
  • Comprehensive service
    Creative Biolabs offers a comprehensive single B cell antibody discovery service that covers all aspects of antibody development, from B cell isolation to antibody generation and optimization. This ensures that the resulting monoclonal antibodies are of high quality and meet the specific needs of each customer.
  • Advanced technologies
    Creative Biolabs uses the latest technologies, such as single-cell omics technologies, to ensure the identification and isolation of B cells with desirable antibody properties. This ensures that the resulting monoclonal antibodies have high specificity and affinity for the target antigen.

What We Can Offer

  1. Customized Workflow
    At Creative Biolabs, we understand that each project is unique, and we work closely with our clients to design a customized workflow that meets their specific needs. Our team of experts will guide you through the process, from sample preparation to antibody characterization, to ensure that you achieve the best possible results.
  2. High-Throughput Screening
    Our Single-B technology enables the screening of millions of B cells in a single run, providing unparalleled throughput and efficiency. This allows for the rapid identification of novel antibody candidates, accelerating the drug discovery process.
  3. Comprehensive Characterization
    We conduct extensive characterization of our antibody candidates to ensure their efficacy and specificity. Our comprehensive characterization services include ELISA, western blotting, surface plasmon resonance (SPR), and functional assays.
  4. High-Quality Results
    Our streamlined workflow ensures the generation of high-quality antibody candidates with high specificity and affinity. We use cutting-edge technologies to isolate single, viable B cells, amplify their antibody genes, and analyze their sequences with bioinformatics tools.
  5. Customizable Services
    At Creative Biolabs, we offer a range of customizable services to meet the specific needs of our clients. We work closely with our customers to design a workflow that is tailored to their unique requirements, providing personalized support and guidance throughout the project.

Service Specifications

Stages Service details Deliverables Turnaround time
Immunization protocol design and antigen preparation (optional)
  • Antigen expression (optional)
  • Antigen detection
/ 2-3 months
Animal immunization Mouse or rabbit /
Isolation of single B cell Round 1 antigen binding screening /
Sequencing of single B cell RT-PCR or sequencing Guaranteed delivery of 20 unique sequences
High-throughput mammalian expression
  • Supernatant ELISA detection
  • Other functional ELISA (optional)
Binding assay results from supernatant
Antibody purification and identification Antibody purification
  • Biacore affinity testing (optional)
  • Functional cell line development (optional)
  • FACS detection (optional)
Delivery of 0.5-1mg antibody.
  • SDS-PAGE > 95%
  • Endotoxin<1 EU/mg
  • ELISA binding assay
! ! For Research Use Only. Not for diagnostic or therapeutic purposes.