Single Cell Genomics Services
Single cell genomics (SCG) is a rapidly advancing field that allows researchers to investigate individual cells at a genomic level, providing valuable insights into cellular heterogeneity, gene expression, and genetic variation. SCG services provide a comprehensive solution for researchers who want to analyze and interpret the genetic information of individual cells. These services use cutting-edge technologies, such as microfluidics, to isolate single cells and generate high-quality genomic data. They offer a wide range of applications, including identifying rare cell types, characterizing tumor heterogeneity, and studying complex developmental processes. With the increasing availability of single cell genomics sequencing services, researchers can accelerate their research and uncover novel insights into the underlying biology of complex biological systems.
Fig.1 Illustration of differences between bulk sequencing vs. single cell sequencing. (Yu, 2020)
Applications of SCG
- SCG in cancer.
Tumor initiation, maintenance, and evolution are facilitated by the successive procurement of genetic variance in single cells. SCG approaches have the capacity to resolve multifaceted blends of cells in tumors. SCG has an insightful impact on our understanding of cancer cell types. Through SCG states in cancer cells and additional cell types, we can uncover rich cell-type heterogeneity and gene-modules activity. This is becoming predominantly important as molecular assays are carried out for directing targeting therapy.
Fig.2 Functional and non-functional intra-tumor heterogeneity in tumor evolution. (Black, 2021)
- SCG in neurobiology
One of the major unanswered questions in neurobiology is whether there is genomic variability between individual neurons of the brain, contribution to the functional diversity, or to an unexplained load of neurobiological diseases. Single cell sequencing methods have provided great insight into questions of neurobiology that have previously been difficult to resolve in genomic data from bulk tissue samples.
Fig.3 Brain regions involved in the components of mindfulness mediation. (Tang, 2015)
- Tissue mosaicism
It was believed that single normal cells have identical genomes, but this dogma is being challenged by increasing confirmation of genetic mosaicism in normal tissue arising in the course of the normal development of the individual. Single cell sequencing has identified variations involved in single cells of normal tissues at an exceptional genomic tenacity. Various studies have been carried out to detect de novo mutation rate and recombination map in single human sperm.
Fig.4 Types of mosaicism and patterns of cutaneous mosaicism. (Biesecker, 2013)
- Immunology
The immune system of humans is largely classified into innate and adaptive modules, which involve a large variety of cell types. SCG has emerged as a powerful tool for studying individual immune cells, which makes possible the observation of rare and intermediate cell states that cannot be resolved at the population level.
Fig.5 Innate and adaptive lymphocyte subsets. (Gasteiger, 2014)
Creative Biolabs' Single Cell Genomics Services
Single Cell Whole Genome Sequencing Service
Creative Biolabs also provides plate-based single cell whole genome sequencing for rare cell samples (e.g., CTCs, circulating tumor cells), as well as for structural variation detection needs where genome-wide coverage is desired.
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Single Cell Target DNA Sequencing Service
Creative Biolabs provides microdroplet-based single cell target DNA sequencing, which enables high-throughput analysis of specific gene regions of interest. This approach is ideal for exploring cell populations and uncovering genetic variation at the single cell level. It is important to note that this service must specify a detection panel.
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Single Microbe Genome Sequencing Service
Creative Biolabs introduces a revolutionary single bacteria DNA sequencing platform. Achieve high-throughput microbial genome sequencing with unmatched resolution. Our technology enables strain-level identification, horizontal gene transfer analysis, and uncovers uncultured strains. Dive deep into microbial genomics with precision and efficiency. Choose Creative Biolabs for unparalleled insights into the microbial world, one cell at a time.
Learn moreCreative Biolabs offers a comprehensive range of single cell genomics services, including whole genome sequencing for detailed genomic profiling, targeted DNA sequencing for specific gene analysis, and single microbe genome sequencing for high-resolution microbial studies. These services provide vital insights into genetic diversity and heterogeneity, aiding in the understanding of complex biological systems and microbial genomics with precision and efficiency.
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References
- Yu, X.; et al. Statistical and bioinformatics analysis of data from bulk and single-cell RNA sequencing experiments. in: Markowitz, M. (eds) Translational bioinformatics for therapeutic development. Methods in Molecular Biology. 2020, 2194: 143-175.
- Black, J.R.M.; et al. Genetic and non-genetic clonal diversity in cancer evolution. Nature Reviews Cancer. 2021, 21: 379-392.
- Tang, YY.; et al. The neuroscience of mindfulness meditation. Nature Reviews Neuroscience. 2015, 16: 213-225.
- Biesecker, L.; Spinner, N. A genomic view of mosaicism and human disease. Nature Reviews Genetics. 2013, 14: 307-320.
- Gasteiger, G.; Rudensky, A. Interactions between innate and adaptive lymphocytes. Nature Reviews Immunology. 2014, 14: 631-639.
