Single Cell Omics Solutions for Circulating Tumor Cells

Catalogue

Empowered by the unmatched expertise in single-cell genomics, transcriptomics, epigenomics and proteomics, Creative Biolabs provides high-quality and innovative single-cell services to our worldwide customers. In addition, our end-to-end single cell sequencing and analysis solutions enable our customers to accelerate their single circulating tumor cell research.

Why Need Single Cell Omics Analysis for Circulating Tumor Cells?

In recent years, single-cell omics data of circulating tumor cells (CTCs) revealed a high degree of cellular heterogeneity in the CTC population, reflecting the heterogeneity across different patients and the potential heterogeneity of tumors within an individual patient. These analyses have enabled the identification of heterologous resistance mechanisms that exist in the treatment of refractory tumors. The existence and extent of heterogeneity revealed by CTCs single-cell omics analysis have important clinical implications for understanding and predicting the development of therapeutic resistance in various solid tumors and for developing appropriate therapeutic strategies for the effective treatment of cancer.

Single Cell DNA Sequencing for CTCs

Single-cell DNA sequencing (scDNA-seq) is a promising method that can improve many facets of tumor research, which enable the sequencing of individual cancer cells, providing the highest-resolution of the mutational histories of cancer. scDNA-seq for CTCs can provide a comprehensive analysis of tumor heterogeneity through the generation of large-scale quantitative genomic datasets. At Single Cell, we offer a wide range of services to meet your single-cell DNA sequencing needs, including cell isolation, DNA extraction and amplification, sequencing library preparation, sequence alignment, and data analysis. Our single-cell DNA sequencing categories include:

• Single cell whole genome sequencing (scWGS) for CTCs
• Single cell whole exome sequencing (scWES) for CTCs
• Single cell targeted sequencing for CTCs

Single Cell RNA Sequencing for CTCs

Single-cell RNA sequencing (scRNA-seq) allows transcriptome analysis of individual cells. With the development of scRNA-seq, it is now possible to discover transcriptome changes, alternative splicing and single nucleotide variation in an individual CTC, explore detailed mechanisms of tumor metastasis, drug resistance, and cancer stemness. Moreover, scRNA-seq can also be used to study tumor heterogeneity and tumor microenvironment. For example, one study analyzed prostate cancer CTC with scRNA-seq technology, showing significant intra-patient heterogeneity for the expression of AR splice variants (ARv7) associated with resistance against anti-androgen treatment. With our extensive experiences in single-cell sequencing, we can provide you with unparalleled scRNA-seq services to accelerate your cancer research.

Single Cell Methylation Sequencing for CTCs

Epigenetics contributes to molecular mechanisms leading to tumor cell transformation and systemic progression of cancer. DNA methylation acts as a major epigenetic mechanism within the complex network of mechanisms regulating gene expression. It is likely to play a key role in CTCs survival and regulation of their metastatic potential. Single Cell has established a robust single cell methylation sequencing platform that allows our clients to explore the epigenetic heterogeneity of tumor cells and CTCs at the single-cell level. We provide a variety of methods, such as bisulfite conversion followed by sequencing (BS-seq), whole-genome bisulfite sequencing (WGBS), single-cell reduced representation bisulfite sequencing (scRRBS) and single-cell post-bisulfite adaptor tagging (scPBAT), which can be used for single cell methylation analysis. In addition, we have developed various methods for analyzing different epigenetic changes at single-cell level.

Single Cell Proteomic Analysis for CTCs

There are many layers to deciphering intercellular heterogeneity. Since there is no direct 1:1 conversion of mRNA generation to protein translation, single-cell proteomics analysis can offer information about the last layer of inter- and intratumoral heterogeneity. At Single Cell, we have state-of-the-art platforms and advanced equipment, which enable us to provide our customers with unrivaled single-cell proteomic analysis services. Our single-cell proteomic analysis services include single-cell barcode chips (SCBCs) analysis, microfluidic image cytometry (MIC) analysis, micro-engraving analysis, single-cell western blotting (scWB) analysis, single-cell time-of-flight and mass cytometry (CyTOF) analysis etc. Please reach out to our scientists for specific assistance.

Highlight

• End-to-end solutions for single cell omics analysis
• The best quality with competitive price
• Expert technical support, full confidentiality, and on-time delivery
• Interdisciplinary efforts across scientists from various fields

With a wealth of experience in single-cell omics analysis, Creative Biolabs can provide you with comprehensive and customized solutions for single-cell programs. We are dedicated to providing new avenues to advance and accelerate your cancer research. Please feel free to contact us for more details.