Single Cell Omics Platforms

Single Cell Sorting and Isolation Platform

Though there is apparent synchrony in cellular systems, analyzed single cell results display that even the same cell or tissue may present different genomes, transcriptomes, proteomes, and epigenomes during cell division and differentiation. Therefore, the sorting and isolation of a single cell are significant for biomedical analysis and further diagnostics, biotechnological applications. Here, we'd dedicated to facilitating the precise selection of single cells in the suspension and ensure our clients to perform powerful and valuable molecular analysis of a specific cell using the following methods.

Single Cell Genomics Platform

Single cell sequencing technology has been developed to dissect the genotypic and phenotypic heterogeneity of a single cell and promises to deepen current understanding of underlying mechanisms regarding both health and disease. Today, we offer a variety of single cell genome-wide approaches to make a unique opportunity to measure different molecules, including DNA, RNA, protein, and chromatin with a final resolution. By isolating these molecules from a single cell simultaneously using our advanced platforms and technologies, it's feasible to profile different types of essential molecules in parallel within the unicellular genome.

Single Cell Transcriptomics Platform

Single cell transcriptomics is a next-generation sequencing (NGS) technology that can simultaneously measure the mRNA concentrations of thousands of genes in single cells. We have established a series of single cell transcriptomics platforms to enable customers to gain a high-resolution view of cells to better understand single cell functions and unravel heterogeneous cell populations in the body. Here are our new approaches to support transcriptomics research:

• Assay for transposase-accessible chromatin using sequencing (scATAC-seq)
• A plate-based 3´-sequencing method based on a "split-and-pool" appraoch (sciRNA-seq)
• Fixed and recovered intact single cell RNA (FRISCR)

We have established a comprehensive series of platforms to support your single cell research.

• Single Cell RNA Sequencing Platform
• Single Cell Gene Expression CRISPR Screening Platform
• Single Cell Surface Protein Characterization Platform
• Single Cell Immune Profiling Platform
• Single Cell Spatial Gene Expression Profiling Platform

Single Cell Epigenomics Platform

Epigenetics involves the study of regulatory systems, including chemical modifications to DNA and relevant histone proteins, as well as changes in DNA accessibility and chromatin conformation. Single cell epigenomics combines sensitive epigenetic profiling, single-cell isolation, barcoding, and high-throughput sequencing (HTS) to describe epigenetic landscapes across cohorts of single cells.

HTS has revolutionized the field of epigenetics with techniques for genome-wide mapping of DNA methylation, histone modifications, and chromatin conformation. Notably, we're dedicated to a wide range of single cell epigenomics analysis services including:

• Analysis of single-cell DNA modifications - Single-cell genome-wide CpG island (CGI-seq) methylation sequencing, single-cell reduced-representation bisulfite sequencing (RRBS), single-cell bisulfite sequencing (BS-seq)
• Single Cell Histone Modification Sequencing Platform - Chromatin immunoprecipitation sequencing (ChIP-seq), single-cell deoxyadenosine methylase ID (DamID)
• Single Cell Chromatin Accessibility Sequencing Platform - ScATAC-seq, DNase I-based single-cell sequencing (scDNase-seq), nucleosome occupancy and methylome sequencing (NOMe-seq), single-cell chromatin overall omic-scale landscape sequencing (scCOOL-seq)
• Single Cell Chromatin Conformation Capture Sequencing Platform - HiC technology (for genome scale)

Single Cell Proteomics Platform

Proteins bridge genetic information and cellular biological functions. Proteomics is of particular interest as it is closely associated with a dynamic picture of cellular behaviors and phenotypic identities. Capturing proteomic information from a single cell has proven to be a substantial technical challenge and the development of such tools requires highly efficient isolation and processing of very large quantities of single cells. As reported, techniques such as microfluidics are extremely useful.

For single cell proteomics, we provide a number of high-sensitivity platforms based on new ideas and emerging technologies, which have promoted the revolution of single cell proteomics enabling the comprehensive understanding of cell functions. Our services allow quantifying thousands of proteins in very small samples, down to single cells.

Single Cell Metabolomics Platform

Single cell metabolomics provides much penetration to clarify cellular heterogeneity of metabolite generation under specific conditions at particular time intervals. This study also has the potential to offer holistic and extensive insights for elucidating organisms' responses under different pathophysiological conditions. Several modern techniques, such as improved ionization methods, high-resolution imaging, and other sensitive techniques are leading the way for single cell metabolomics.

As below, we highlight some of the common categories of microfluidic approaches for single cell metabolomics, including fluorescence-mediated analysis, electrochemical assays, MS, and so on.