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Single Cell Functional Analysis Services

The physiological or pathological status of an individual, genetic specificities, age, diet, lifestyle, and environment all influence the interactions between a multitude of actors inside and outside the cell and within different cells, which cooperate under the influence of an individual's physiological or pathological status, age, diet, lifestyle, and environment. Standard technologies for cell analysis employed in clinical studies are a vital source of information in biomedical and clinical research, but they are primarily limited to cell cluster analysis. However, within the same isogenic population, individual cells can have substantial differences, which can impact the health and cellular function of the entire population.

Our Single Cell Functional Analysis Services

Tumor cell metastasis.

Single cell motility and migration analysis service

Single cell motility and migration analysis can rule out cell-cell adhesion and proliferation, which affect wound healing and Boyden chamber assays. Cells should be plated immediately after being removed from the culture, unlike wound-healing or Electric Cell-substrate Impedance Sensing (ECISTM) tests, which require confluence. Seeding cells before time-lapse microscopy lets researchers control extracellular matrix (ECM) components that affect migration studies.

With automated microscope stages, processing power, and data storage, massive amounts of picture data can be generated automatically or semi-automatically. Imaging research is slowed by data analysis, rather than capture. Computer-aided image analysis, a semi-automated quantitative method, can quickly handle complex processes like cell behavior. Manual cell speed tracking is the industry standard.

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Cell cycle.

Single cell division and growth analysis

Cells are available in a variety of sizes and shapes. The smallest bacteria have cell dimensions of several hundred nanometers, while the largest bacteria have cell dimensions of several hundred micrometers. Typically, eukaryotic cells are larger than bacterial cells, with sizes ranging from a few micrometers to meters for nervous system cells. However, all cells have a size range within which they function optimally, and size control systems can assist in keeping them within that range. Thus, cell growth and division could be coordinated to maintain cell size homeostasis over time.

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Stages of apoptosis in cancer cells.

Single cell death analysis service

Cell death is crucial in the development of organisms, the etiology of disease, and cell responses to treatments. Apoptosis is a type of programmed cell death involving caspases, which are specialized cysteine proteases found in animal cells as inactive proenzymes. Apoptosis is a classic systems-level challenge in which complicated circuits are composed of graded and competing chemical signals dictating binary life-death decisions at the single-cell level. Clinically, diseases such as cancer disrupt the natural balance of cell proliferation and cell death, and anticancer medications are thought to work by inducing apoptosis in cancer cells.

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Synaptic cleft.

Single cell cellular communication analysis service

Inter- and intracellular communication is a complex, multi-effector-driven process. Signaling proteins, the key players in cellular communication, are shape- and behavior-variable. They are capable of interacting with multiple molecular components and incorporating numerous types of cellular data. Signal transduction is one of the primary cellular communication processes. Specifically, G-protein signaling modulates an abundance of responses.

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Creative Biolabs offers services for the functional analysis of single cells, including analyses of single cell motility and migration, single cell division and growth, single cell death, and single cell cellular communication. These services for analyzing cellular function at a single-cell resolution can provide a new and more comprehensive perspective on cellular life and function. For any information, please contact us.

! ! For Research Use Only. Not for diagnostic or therapeutic purposes.