Single Cell Cellular Communication Analysis Service
The process of intercellular and intracellular communication is a complex one that is coordinated by a variety of effectors. Signaling proteins, which serve as the primary actors in cellular communication, are changeable in both their form and their function. They are able to engage in interaction with a variety of cellular components and incorporate a wide variety of data kinds. One of the most important aspects of cellular communication is referred to as signal transduction. Specifically, the signaling of G-proteins is the mechanism that is responsible for modifying a wide variety of responses.
Fig.1 Cell communication types. (Tan, 2016)
Single Cell Analyses Using Fluorescent Biosensors
Biosensors detect biological events using optical signals. Genetically encoded fluorescence biosensors have improved cell biochemistry research. A sensor unit detects biological activity and changes the fluorescent signal from a reporting unit in these modular gadgets. Many proteins relocate when binding partners arise or disappear in subcellular locations. Translocation-based biosensors use fluorescence redistribution to detect specific chemicals. A molecular switch controls the reporting unit's fluorescence characteristics in the sensing unit. Proteins or protein fragments that conform to incoming signals are molecular switches. A biosensor's fluorescence intensity varies when a molecular switch is inserted into a single fluorescent protein (FP). A molecular switch can also be linked to a pair of Fluorescence Resonance Energy Transfer (FRET)-capable FPs to transmit excited state energy from a donor fluorophore to a neighboring acceptor. These basic designs are a broad molecular toolset for cell signaling visualization. These biosensors may be directly expressed and targeted throughout the cell, making them effective tools for monitoring biochemical processes at specific subcellular sites, which is useful for investigating signaling dynamics.
Single cell analyses using fluorescent biosensors provide an advantage in directly observing living cells in a real-time manner, gathering both temporal and spatial information on biochemical activities.
Fig.2 Standard approaches for biosensor design. (Clister, 2015)
Creative Biolabs offers a single cell cellular communication analysis service to help researchers study the complex process of cell communication. If you have any requirements, please feel free to contact us for further communication about your project.
- Tan, M. Cell and molecular biology of diagnostic and therapeutic technology. Journal of Physics Conference Series. 2016, 694(1): 012001.
- Clister, T.; et al. Single-cell analysis of G-protein signal transduction. Journal of Biological Chemistry. 2015, 290(11): 6681-6688.