Single Cell Division and Growth Analysis Service
Creative Biolabs offers different types of single cell division and growth analysis to fulfill the needs of clients.
Cell Division and Growth
Cells come in a wide range of sizes and shapes. The smallest bacteria have a cell size of a few hundred nanometers, whereas the largest bacteria have a cell size of a few hundred micrometers. Eukaryotic cells are typically larger than bacterial cells, ranging in size from a few micrometers to meters for nervous system cells. However, all cells have a specific size range within which they function best, and size control systems may help keep them within that range. Cell growth and division activities could thus be coordinated to preserve cell size homeostasis throughout time.
Fig.1. Schematic of three competing cell division control models. (Vuaridel, 2020)
Single Cell Division and Growth in Eukaryotic cells
|Paper Title||Characterization of dependencies between growth and division in budding yeast|
|Journal||Journal of the Royal Society Interface|
|Abstract||This study measures single-cell growth and division of Saccharomyces cerevisiae in 26 wild-type lineages (782 cells) grown in glucose, 19 6× CLN3 lineages (376 cells) grown in glucose and 21 wild-type lineages (518 cells) grown in glycerol/ethanol.|
The presence and disappearance of the myosin ring, which was seen by tagging Myo1p with green fluorescent protein (GFP), were used to track cell-cycle development for each yeast cell on the plate. The myosin ring is a contractile component that forms late in G1 and just before the bud emerges. The red fluorescent protein DsRed, which was placed under the control of the promoter of ACT1, the constitutively produced actin gene, was used to track cell development. Total red fluorescence in a cell was used as a proxy for total protein content or cell mass in this manner. At each time point, the red fluorescence in a cell was measured and appropriately adjusted across all cells in a microcolony.
Fig.2. Single-cell lineages and cell types are depicted and classified. Images of representative cells going through cell-cycle events are arranged along each branch of the lineage tree. A mother cycle is indicated by binary cell labels that finish in 0, whereas a daughter cycle is indicated by binary cell labels that end in 1. (Mayhew, 2017)
Single Cell Division and Growth in Bacterial cells
|Paper Title||Cell division in Escherichia coli cultures monitored at single cell resolution|
|Abstract||The ability to divide is one of the most basic characteristics of cells. Most bacterial culture characteristics, including cell division, have been measured as cell population averages assuming that all bacteria divide at the same rate.|
They monitored the division of single cells in Escherichia coli cultures during different growth phases. Their experiments are based on flow cytometry monitoring of green fluorescent protein (GFP) dilution during cell division. The results show that in exponentially increasing cultures, the vast majority of E.coli cells divide equally. No cell division was seen in cultures that had been in the stationary phase for up to four days. However, when the stationary phase culture was diluted into fresh medium, two subpopulations of cells emerged: one that began to divide and one that did not. GFP dilution was used to detect these populations, and flow cytometry revealed varied side scatter characteristics.
Fig.3. Schematic representation of the GFP dilution experiment. HSL is used to promote GFP expression. The inducer is removed once the cells have accumulated GFP by centrifuging them and putting them in new (or conditioned) media. The amount of GFP in growing cells (A) decreases with each cell division. The amount of GFP in non-growing cells (B) remains constant. (Roostalu, 2008)
With years of experience, Creative Biolabs has gained remarkable success in single cell division and growth analysis. Using our unique and accessible approach to live-cell imaging and analysis, you can gain a better understanding of therapy impacts on cell cycle dynamics. Please contact us for more information. Our experts will help design an optimal solution for your project and trouble-shoot for you throughout the whole process.
- Vuaridel, G., et al. Computational analysis of the mutual constraints between single-cell growth and division control models. Advanced biosystems. 2020; 4(2): 1900103.
- Mayhew, M.B., et al. Characterization of dependencies between growth and division in budding yeast. Journal of the Royal Society Interface. 2017; 14(127): 20160993.
- Roostalu, J., et al. Cell division in Escherichia colicultures monitored at single cell resolution. BMC microbiology. 2008; 8(1): 1-14.