Single Cell Multi-Omics Services

Creative Biolabs is a good partner of pharmaceutical companies and research institutions. We provide single cell multi-omics services to accelerate your project progress.

Single Cell Multi-Omics

Multi-omics analyses have been shown to be useful in gaining a thorough understanding of biological activities. This advantage has facilitated the development of single cell multi-omics analysis, which allows researchers to investigate cell type-specific gene regulation. (1) single cell isolation, barcoding, and sequencing technologies to measure multiple types of molecules from the same cells, and (2) integrative analysis of the molecules measured at the single cell level to identify cell types and their functions related to pathophysiological processes based on molecular signatures are the core components of single cell multi-omics analysis.

Fig.1 Strategies for multi-omics profiling of single cells. (Hu, 2018)

Analysis of Single Cell Multi-Omics Data

Multi-group analysis can quickly collect a significant amount of data. By merging the research findings with the key analysis, the results can be generated rapidly. The data from omics at various levels may be cross-verified, making the experiment's conclusion gained through extensive analysis more credible. Multi-layer omics integration can compensate for the shortcomings of single-layer omics in detecting preference differences. The fault induced by the difference in preference in single group detection can be compensated for by multilayer omics integration. It is simpler to discover the difference by evaluating downstream omics data, as there is a propensity to intensify the influence from gene to protein to metabolism.

The approaches developed for single cell mono-omics data have been extended and merged for the integrative analysis of single cell multi-omics data. The methodologies are: (1) single-cell mono-omics data correlation analysis; (2) analysis of one kind of single cell data followed by integration of another single cell data type; and (3) integrative analysis of all types of single cell omics data to generate the overall single cell map.

Fig.2. Strategies for the integrative analysis of single cell multi-omics data. (Lee, 2020)

Applications of Single Cell Multi-Omics

• To identify cell subtypes from a heterogeneous cell population
• To reconstruct cell lineage trajectories
• To definitively reveal the relationship between different omics readouts

Advantages of Our Multi-omics Service

• In-depth investigation of potential pathogenic variables: including integrating genomes and transcriptomics data and analyzing the multi-layer response to disease onset
• Construct a gene regulatory network: A network link exists between genes, mRNA, regulatory factors, and proteins in an organism. The numerous chemicals are linked together through the creation of gene regulatory networks.
• Multi-omics analysis: For example, the influence of candidate genes screened at the DNA level on transcription, mutation analysis at the RNA level for genes that are considerably differentially expressed at the RNA level, and integrated analysis based on metabolite related enzymes and differential genes.

Creative Biolabs can customize exclusive solutions for you based on your projects and needs. Please contact us for more information about our single cell multi-omics services. Our experts will help design an optimal solution for your project and trouble-shoot for you throughout the whole process.

References

1. Hu, Y., et al. Single cell multi-omics technology: methodology and application. Frontiers in cell and developmental biology. 2018; 6: 28.
2. Lee, J., et al. Single-cell multiomics: technologies and data analysis methods. Experimental & Molecular Medicine. 2020; 52(9): 1428-1442.