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Creative Biolabs can provide one-stop solution for single cell COOL-seq service that fundamentally reveal the differences in types and their states between different individual cells, allowing you to better understand cells and their phenotypes and life activities.

Single Cell COOL-seq

Because of cell heterogeneity, single cell analysis is becoming increasingly popular in life science and precision medicine. The rise of single cell omics with high sensitivity, throughput, and multiplexity has been re-energized by recent significant improvements in analytical platforms and tools. The omics information promotes a deeper understanding of disease development processes by bridging the gap between underlying molecular alterations and cell behavior. Single cell COOL-seq consists mostly of genomics, epigenomics, transcriptomics, proteomics, and metabolomics, and is used in stem cell differentiation, immune cell function, nerve cell development and activity, and cancer research based on circulating tumor cells.

Schematic illustration of the assay designs and biomedical applications of single cell omics.Fig.1. Schematic illustration of the assay designs and biomedical applications of single cell omics. (Chen, 2020)

Our Single Cell COOL-seq Services

  • Cell Isolation

    Single cell isolation that is both efficient and high-throughput serves as a preconditioner for single cell omics. Cell isolation platform in Creative Biolabs have been developed mainly including micromanipulation, FCM, laser capture microdissection (LCM), and microfluidics.

  • Genomics and Epigenomics

    Single cell genomics includes whole-genome amplification (WGA), DNA sequencing considerations, and sequence data analysis. single-cell epigenome sequencing assays include RRBS-seq, ChIP-seq, ATAC-seq, DNase-seq, FAIRE-seq and etc.

  • Transcriptomics

    Transcriptomics has become a potent technique for linking cell genotype to phenotype with the emergence of single-cell RNA sequencing (scRNA-seq) technology, which can disclose information about gene expression status and gene structure at the overall level within a single cell.

  • Proteomics

    Techniques including as microscopy, FCM, MS, and mass cytometry (CyTOF) have been widely used in single-cell proteomics analysis.

  • Metabolomics

    MS has emerged as the most commonly used method at the single-cell metabolomics, as it is a label-free technique that can rapidly analyze hundreds of metabolites simultaneously.

Applications of Single Cell COOL-seq

Single cell COOL-seq service can help researchers get insight into stem cells differentiation, immune cells function, nerve cells development and activity, and have a wide range of applications in the fields of cancer research.

Applications of single cell COOL-seq.Fig.2. Applications of single cell COOL-seq.

Published Data

Paper Title Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells
Journal Cell research
Published 2017
Abstract In this study, they developed a single-cell multi-omics sequencing method (single-cell COOL-seq) that can examine chromatin state/nucleosome positioning, DNA methylation, copy number variation, and ploidy from the same mammalian cell at the same time. They used this technology to look at how chromatin state and DNA methylation were reprogrammed in mouse preimplantation embryos. They discovered that each individual cell undergoes worldwide genome demethylation within 12 hours of fertilization, as well as rapid and global reprogramming of both maternal and paternal genomes to a highly open chromatin state. After the late zygote stage, there was a decline in openness.
Methods Single-cell COOL-seq
Results With high sensitivity and coverage, single-cell COOL-seq technology can analyze chromatin accessibility, nucleosome positioning, DNA methylation, CNV, and ploidy from the same individual cell. This is the first time that different layers of epigenetic regulations can be determined concurrently in the same single cell. They can detect both chromatin openness and endogenous DNA methylation levels in the promoter regions of the majority of RefSeq genes (75.3 percent, 18 337 out of 24 346) in a single ES cell. From a single ES cell, they can identify both chromatin openness and endogenous DNA methylation levels for the majority (70.3 percent, 11 246 out of 15 991) of the CpG islands.

Establishment of single-cell COOL-seq in mouse embryonic stem cells. Fig.3. Establishment of single-cell COOL-seq in mouse embryonic stem cells. (Guo, 2017)

Creative Biolabs offers exclusive solutions for you according to your specific project requirements. If you want to know more about single-cell COOL-seq service, please feel free to contact us. We’re dedicated to advancing the success of your every project.


  1. Chen, W., et al. Single cell omics: from assay design to biomedical application. Biotechnology Journal. 2020; 15(1): 1900262.
  2. Guo, F., et al. Single-cell multi-omics sequencing of mouse early embryos and embryonic stem cells. Cell research. 2017; 27(8): 967-988.
! ! For Research Use Only. Not for diagnostic or therapeutic purposes.