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Creative Biolabs offers single cell triple omics sequencing service to analyze genomic CNVs, the DNA methylome, and the transcriptome of an individual mammalian cell simultaneousl. With extensive experience and expertise, our single cell triple omics sequencing service can understand the cell subtypes and cell lineage trajectories, which enables deeper insights into gene regulatory programs that can investigate the complexities of diseases.

Single Cell Triple Omics Sequencing

Methods for sequencing the single-cell genome, DNA methylome, and transcriptome have all been developed separately. However, these omic approaches must be done in the same single cell to precisely investigate the process by which the transcriptome, genome, and DNA methylome regulate each other. The single-cell triple omics sequencing technique (scTrio-seq) examines the genomic copy-number variations (CNVs), DNA methylome, and transcriptome of a single mammalian cell at the same time. The scTrio-seq method is unique in that it computes both DNA methylation and copy number states from scRRBS sequencing libraries while also measuring cytoplasmic transcript levels from the same single cell. The methylome sequences of scM&T-seq and scMT-seq data may theoretically be used to mine DNA copy number landscapes, although this has yet to be proved.

A flow chart illustrating the scTrio-seq technique.Fig.1. A flow chart illustrating the scTrio-seq technique. (Hou, 2016)

Our Single Cell Triple Omics Sequencing Service

We offer scientific and meticulous design for our single cell triple omics sequencing service to ensure high-quality research results, including sample preparation, library construction, sequencing and data analysis. The quality of the final data collection is jeopardized if any of these procedures produce poor results. Library construction is an especially crucial step. Our library construction technology can help assure excellent sample quality and accuracy for any research project.

Our single cell triple omics sequencing service.Fig.2. Our single cell triple omics sequencing service.

Application of Single Cell Triple Omics Sequencing

scTrio-seq can explore the relationship between the genome (CNVs), DNA methylome, and transcriptome in a single cell. The role of epigenetic changes in controlling chromatin state and gene expression is critical. They can be heterogeneous within a cell population, which is especially true in cancer. DNA methylation in the promoter regions frequently adversely correlates with gene expression, but DNA methylation in the gene body often positively corresponds with gene expression, according to numerous studies.

Published Data

Paper Title Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas
Journal Cell research
Published 2016
Abstract In this study, they present scTrio-seq, a single-cell triple omics sequencing technique that can be used to analyze the genomic copy-number variations (CNVs), DNA methylome, and transcriptome of a single mammalian cell simultaneously. They show that large-scale CNVs generate proportional changes in gene RNA expression inside the acquired or lost genomic regions, but that these CNVs have no effect on DNA methylation in these areas. They also used scTrio-seq to analyze 25 single cancer cells taken from a human hepatocellular carcinoma tissue sample. Based on CNVs, DNA methylome, and transcriptome of individual cells, they found two subpopulations within these cells.
Methods Single-cell triple omics sequencing
Results They can find subpopulations of cancer cells based on genome (CNV) information using scTrio-seq, then infer malignancy and metastatic potentials of the subpopulations based on triple-omic data. They can also investigate the connections between differential CNV areas, differentially expressed genes, and DMRs. They can reveal the heterogeneity within each subpopulation after filtering out differences between subpopulations. By simultaneously investigating the genome, methylome, and transcriptome of their constituents at the single-cell level, they pave the way for unraveling the heterogeneity and complexity of cell populations in development and cancer.

Differences in triple omics between subpopulation I and II of HCC cells. Fig.3. Differences in triple omics between subpopulation I and II of HCC cells. (Hou, 2016)

Creative Biolabs provides a one-stop service for single cell triple omics sequencing services, which aims to conduct a comprehensive analysis of single cell. Please contact us for more information about our single cell triple omics sequencing services.

Reference

  1. Hou, Y., et al. Single-cell triple omics sequencing reveals genetic, epigenetic, and transcriptomic heterogeneity in hepatocellular carcinomas. Cell research. 2016; 26(3): 304-319.
! ! For Research Use Only. Not for diagnostic or therapeutic purposes.

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