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Accelerating chromatin mapping with single-cell ATAC-seq to make your research better, faster.

Single-cell assay for transposase accessible chromatin sequencing (ATAC-seq) is a powerful tool to interrogate the epigenetic heterogeneity of cells in healthy and diseased tissues, including SARS-CoV-2 infection. Empowered by unrivaled expertise and advances in single-cell approaches, Creative Biolabs provides an open, flexible platform to develop and optimize single-cell ATAC-seq (scATAC-seq) services to study epigenetic heterogeneity of cells/tissues which are associated with virus entry and transmission.

Importance of Single-cell ATAC-seq

Single Cell ATAC-seq for SARS-CoV-2 (COVID-19) Research

Accessible regions within chromatin often contain important genomic elements for transcription factor binding and gene regulation. scATAC-seq profiles the chromatin accessibility map and epigenetic landscape at the single-cell level to gain a deeper understanding of the molecular mechanisms behind healthy and infection processes in the body. Through the scATAC-seq data analyses of healthy and infected cells, we can deeply understand the accessible regions of the genome and subsequently reveal the role of DNA-binding proteins, nucleosomes, and chromatin compaction in regulating gene expression during infection. These new insights will greatly promote the study of relevant protective or pathogenic mechanisms and the discovery of effective therapeutics.

Single-cell ATAC-seq for SARS-CoV-2

It has been demonstrated that SARS-CoV-2 binding via angiotensin converting enzyme 2 (ACE2) and subsequent priming by type 2 transmembrane serine protease 2 (TMPRSS2) protease. ACE2 is shown to present heterogeneous expression within the individual cell subtypes in different organs and different species. To understand whether epigenetic mechanisms underlie the heterogeneity of cellular composition and cell state variation, scATAC-seq can be used to help us understand the sequence variation of ACE2 and changes in the proportions of cell subtypes in organs between species. Through analyses of scATAC-seq data, we can choose a best-fit animal model closest to humans to advance our knowledge of COVID-19. In addition, scATAC-seq has analyzed the open chromatin regions within the ACE2  locus and found the enrichment of transcription factors STAT1, STAT3, and IRF1 binding sites, which are direct targets of tissue protective and innate immune responses such as IL-6 signaling pathway and interferons. This correlation of ACE2 and IL-6 suggests that cytokines, particularly IL6, can exacerbate the disease by aiding viral entry into target cells. These targets may offer therapeutic opportunities for the treatment of COVID-19.

scATAC-seq reveals epigenetic regulation of ACE2 in kidney.Fig.2 scATAC-seq reveals epigenetic regulation of ACE2 in the kidney. (Han, 2020)

At Single Cell, our experienced scientists can provide a fast, one-stop single-cell ATAC-seq solution - from sample through data analysis to reduce your cost and turnaround time. Our scATAC-seq profiles the chromatin accessibility landscape at single-cell level during SARS-CoV-2 infection, thus revealing cell-to-cell variability at the basis of epigenetics. scATAC-Seq solution has also given customers the power to study rare cell populations, opening up new applications such as the ability to study infection heterogeneity or identify rare cell types or potential therapeutic targets.

Except for scATAC-seq, Creative Biolabs also provides tailored scRNA-seq, scTCR-seq, and scBCR-seq services to bring critical insights into the immune clearance mechanism and new therapeutic strategy for COVID-19. Our scientists are dedicated to designing innovative approaches to gain a multidimensional view of your research at single-cell level.

Highlighted Features

  • Highest throughput sequencing platforms improves the data quality and reliability
  • Experienced staff to assist you with experimental design
  • High accuracy and sensitivity to reduce repeats
  • Interactive bioinformatics analysis report

If you are interested in our services, please feel free to contact us.

Reference

  1. Han, L.; et al. Single-cell atlas of a non-human primate reveals new pathogenic mechanisms of COVID-19. bioRxiv. 2020.
! ! For Research Use Only. Not for diagnostic or therapeutic purposes.

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