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Single-cell Provides A Direct View into Virus-Host Interaction to Accelerate Biomarker Discovery.

The nature and the number of proteins reflect differences in cell subpopulations/subsets or cell cycle/differentiation stages, thereby influencing viral replication and pathogenesis, as well as defining the cell susceptibility and permissiveness to viral infection. Empowered by unrivaled expertise and advances in single-cell approaches, Creative Biolabs provides a comprehensive suite of single-cell protein screening services to promote SARS-CoV-2 (COVID-19) research.

The Importance of Single Cell Protein Screening

Single Cell Protein Screening for SARS-CoV-2 (COVID-19) Research

Proteins regulate almost all cellular functions, including providing structure to cells, controlling cell growth and adhesion, functioning as enzymes to catalyze biochemical processes, and regulating signal transduction. It is therefore critical to characterize the quantity and activity of proteins to understand molecular mechanisms of cellular processes, such as infection progression, cell differentiation and fate, and for targeted discovery and development of novel therapeutics, vaccines, and diagnostics.

Conventional protein analysis techniques, such as gel electrophoresis, immunoassays, chromatography, and mass spectrometry, require a large population of cells, resulting in a population-averaged measurement. Population-averaged data may mask the underlying molecular mechanisms because of cellular heterogeneity. Single-cell level screening of proteins has provided valuable insight into mechanisms that dictate heterogeneity in cellular responses to SARS-CoV-2 infection.

Methods for Single Cell Protein Analysis

With the advances in single-cell technology, there are a series of single-cell protein screening methods including single fluorescence-activated cell sorting (FACS), multi-parameter flow cytometry, microfluidics, liquid chromatography coupled to tandem mass spectrometry (LC-MS/MS) have been developed and are still getting optimized. Below is a summary of the distinctive advantages and disadvantages of the current methods.

Single Cell Protein Screening for SARS-CoV-2 (COVID-19) Research

Single Cell Protein Screening Workflow in SARS-CoV-2 Study

With the goal of evaluating the degree of cellular heterogeneity, Creative Biolabs focuses on detecting the expression of specific proteins to reveal the mechanisms underlying viral infections and the interactions between hosts and viruses at the single-cell level. Our comprehensive protein profiling brings valuable insight into studying viral infection in relation to host cellular heterogeneity and exploring cellular heterogeneity, identify rare cell types, novel targets or biomarkers, as well as cell types and states during infection.

Single cell protein screening. Fig.1 Single-cell protein screening.

Single-cell protein screening measures a wide variety of proteins in single cells to reveal cellular heterogeneity to discover novel therapeutic targets or diagnostic biomarkers. To better understand the new SARS-CoV-2 virus and promote basic research and clinical therapy development, scientists at Creative Biolabs offer several different approaches to single-cell protein screening, ensuring that high-quality data meet the right needs. If you have additional requirements or questions, please feel free to contact us.

! ! For Research Use Only. Not for diagnostic or therapeutic purposes.