Transcriptome In Vivo Analysis
For the purpose of comprehending these cellular differences, it is necessary to first capture and then isolate single cells. However, capturing and isolating single cells in an effective manner from any cell population can be a challenging undertaking. As a result, a wide variety of methods for the capture and isolation of single cells have been investigated.
Transcriptome In Vivo Analysis (TIVA)
The TIVA procedure is one method that can be utilized to successfully isolate mRNA from a single cell. We need an mRNA capture technology that not only has a high spatial resolution but is also compatible with living tissue that has not been damaged. This will allow us to study the transcriptomes of single cells while those cells are still in their natural environments.
In the process of isolating mRNA from a single cell inside complex tissues using the TIVA, a photoactivatable mRNA capture molecule referred to as the TIVA tag is utilized. The TIVA approach is the first technology that does not entail any kind of invasive treatment in order to extract mRNA from living single cells while such cells are located in their natural environments.
Fig.1 TIVA tag is an oligonucleotide hairpin capable of penetrating and fluorescently labeling living tissue. (Lovatt, 2013)
Advantages of TIVA
- Cells remain in their natural microenvironment.
- High spatial resolution.
- Compatible with live, intact tissues.
At Creative Biolabs, we can provide TIVA service for a better solution for single-cell transcriptome isolation. If you have any requirements, please feel free to contact us for further communication about your project.
Reference
- Lovatt, D.; et al. Transcriptome in vivo analysis (TIVA) of spatially defined single cell in live tissue. Nature Methods. 2014, 11: 190-196.
