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In the past two decades, some diseases have achieved major research progress. In recent years, single cell research techniques have continued to advance, setting off a wave of research on tumor heterogeneity. Single Cell has established advanced single cell omics platforms to assist you with single cell services and provide you with accurate and comprehensive analysis results through bioinformatics.

Uncover the Heterogeneity of Intratumor Cells

Tumor heterogeneity is a broad definition that can be roughly divided into temporal heterogeneity, spatial heterogeneity, structural heterogeneity, cellular heterogeneity, protein heterogeneity, genetic heterogeneity, functional heterogeneity, etc. In 2011, scientists used DNA sequencing to analyze copy number variation in individual breast cancer cells and inferred cell population structure and tumor evolution. This work initially answered the extent of breast cancer clonal heterogeneity. The mutations identified in tumor cells could serve as therapeutic targets, suggesting that tumor heterogeneity can be used to guide the precision therapy discovery.

Intra-tumor heterogeneity in different cancer types.
Models for circulating tumor cell development and cancer progression.

Uncover the Heterogeneity of Circulating Tumor Cells

Cancer is difficult to treat because of their extensive spread and recurrence. Even though many treatments are available such as surgical resection, radiotherapy, and chemotherapy, the patients' survival rate is still very low. Circulating tumor cells can exit the bloodstream and enter other organs, grow into new tumors, causing metastatic tumor. In order to develop a better treatment plan, it is necessary to conduct a more in-depth study on the molecular level of tumor cells, especially the circulating cells that cause recurrence. Although traditional RNA sequencing can distinguish tumor subtypes, it is impossible to analyze tumor heterogeneity at a single cell level. To better understand the diffusion process of tumor, scientists developed single-cell RNA sequencing to sequence cancer cells and adjacent cancer cells, and successfully distinguished circulating cancer cells. Moreover, the heterogeneity of circulating tumor cells can provide insights for early tumor prediction.

Uncover the Heterogeneity of Tumor Infiltrated Immune Cells

T cells play critical roles in tumor immune microenvironment. Tumor infiltrated immune cells including effector T cells, memory T cells, regulatory T cells, and helper CD4 T cells are in "dysfunctional" or "depleted" states. Unraveling the characteristics of single tumor infiltrated immune cells can provide insights for the mechanism of tumor immune evasion, facilitating the discovery of novel tumor immunotherapies. Scientists performed single-cell sequencing analysis for tumor infiltrated immune cells, and identified novel immune cell types, novel markers, undefined mutations, novel tumor evasion mechanisms, novel anti-cancer targets, etc.

Tumor infiltrated immune cells.
Interaction between microbiome and immunity.

Uncover the Heterogeneity of Immunity Associated Microbiome

Host immunity plays a critical role in regulating clinical responses to various treatment. Recently, the gut microbiome has been demonstrated to play a major role in mediating overall immune set-point, via various effects on local and systemic inflammatory processes. Scientists have found that quantifiable differences in the microbiome are associated with disease and treatment outcomes. However, the detailed mechanisms of immune-microbiome interactions have not been clarified. Single cell technologies provide a powerful tool to investigate and explore the heterogeneity of immunity associated microbiome.

Our Highlights

  • High throughput: Thousands of single cells
  • Personalization: Analysis can be customized to fit your specific requirements
  • One-stop-shop: One-stop service from sample preparation to data analysis

Creative Biolabs has developed the most strategic approaches to provide you with comprehensive suite of single-cell omics solutions. We are dedicated to offering end-to-end single-cell multi-omics solutions to advance your project and maximize your success. To explore more about our services, please contact us for assistance.

References

  1. Sun, X.; et al. Intra-tumor heterogeneity of cancer cells and its implications for cancer treatment. Acta Pharmacologica Sinica. 2015, 36: 1219-1227.
  2. Magbanua, M.J.M.; et al. Approaches to isolation and molecular characterization of disseminated tumor cells. Oncotarget. 2015, 6(31): 30715-30729.
  3. Lint, S.Van.; et al. Priming of Cytotoxic T Lymphocyte Responses by Dendritic Cells: Induction of Potent Anti-tumor Immune Responses. Horizons in Cancer Research. 2013, 51.
  4. Mezouar, S.; et al. Microbiome and the immune system: From a healthy steady-state to allergy associated disruption. Human Microbiome Journal. 2018, 10: 11-20.
! ! For research purposes only. Not for clinical, therapeutic, or diagnostic purposes in animals or humans.

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