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The new era of quantitative cell imaging—challenges and opportunities

The New Era of Quantitative Cell Imaging—challenges and Opportunities

Quantitative optical microscopy—an emerging, transformative approach to single-cell biology—has seen dramatic methodological advancements over the past few years. However, its impact has been hampered by challenges in the areas of data generation, management, and analysis. Here we outline these technical and cultural challenges and provide our perspective on the trajectory of this field, ushering in a new era of quantitative, data-driven microscopy. We also contrast it to the three decades of enormous advances in the field of genomics that have significantly enhanced the reproducibility and wider adoption of a plethora of genomic approaches.