Single Cell Omics Solutions for Dendritic Cells (DCs)

Creative Biolabs is one of the world's leading providers of single-cell technologies, including single-cell DNA sequencing (scDNA-seq), single-cell RNA-sequencing (scRNA-seq), single-cell methylation sequencing (scMethyl-Seq), single-cell proteomic analysis etc. With a wealth of experience and expertise in the field of single cell analysis, we offer comprehensive single-cell omics solutions and technical services to our customers. These comprehensive services are designed to be one-stop and we can give the results you need to advance your biological and biomedical research.

Overview of Single Cell Omics in Dendritic Cells (DCs)

DCs are specialized antigen-presenting cells that initiate T cell-mediated immune responses and function as key mediators of immunity and tolerance. DCs are heterogeneous and consist of multiple subtypes. Numerous studies have shown that human DC subtypes in the blood include CD11c⁺ conventional DCs (cDCs), consisting of either CD141⁺ or CD1c⁺ cells, and plasmacytoid DCs (pDCs). However, it is unclear how many DC subtypes exist, how they are related to each other and how they differ from other mononuclear phagocytes. The recent emergence of single-cell omics technologies, especially scRNA-seq, affords a direct means of identifying and fully characterizing functionally important subsets of DCs and their complex underlying biology roles. Most importantly, scRNA-seq is used to characterize and study heterogeneity across cell types, development, and dynamic processes in the immune system.

Our Single Cell Omics Solutions for Dendritic Cells (DCs)

Single-cell analysis has become a powerful method to reveal cell heterogeneity, subpopulation diversity, and rare cell characterization etc. At Single Cell, we provide comprehensive single cell omics solutions including scDNA-seq, scRNA-seq, scMethyl-Seq, scProteomic analysis to profile DCs, a critical role in immune system. In addition, our experts can provide customized single-cell omics solutions tailored to fit your specific needs at single-cell level. From our single cell omics solutions, you can identify novel cell types and characterize the cellular composition or heterogeneity in healthy and diseased tissues. We bring creative minds together for a common goal: accelerating the progress of our clients' programs within the desired timeline and budgets.

We are dedicated to providing a full spectrum of single cell analysis for DCs. Here are 2 examples using scRNA-seq technology to characterize the heterogeneity of DCs for your reference.

Example 1: Revealing the heterogeneity of pre-cDC by scRNA-seq

In humans, cDCs exist in two distinct populations characterized by expression of CD1c and CD141. cDCs arise from bone marrow (BM) progenitors that include the common DC progenitor that differentiates into the pre-cDC, the direct precursor of cDCs. Although pre-cDC has the potential to differentiate into CD1c⁺ and CD141⁺ cDCs, most pre-cDCs appear to be committed to differentiation into one or the other subset. To determine if there are two distinct precursor populations of differentially regulated cDCs in humans that are committed to either the CD1c⁺ or CD141⁺ cDC fate, Gaëlle et al. studied the transcriptomic characteristics of single pre-cDCs by scRNA-seq, and the results showed that human pre-cDCs are heterogeneous, consisting of two different precursor populations that are precommitted to become either CD1c⁺ or CD141⁺ cDCs. The two groups of lineage-primed precursors can be distinguished according to the differential expression of CD172a. Both subpopulations of pre-cDC appear in adult BM and can be found in cord blood and adult peripheral blood.

Fig.1 Heterogeneity in human pre-cDCs revealed by scRNA-seq. a) Gating strategy for isolation of CD1c⁺ and CD141⁺ cDCs in blood. b) Unsupervised clustering reveals transcriptomic characteristics of single differentiated blood cDCs. c) Gating strategy for isolation of human pre-cDCs in cord blood. d) Single-cell clustering of single cord blood pre-cDCs. (Gaëlle, 2016)

Example 2: Identification of DC subtypes by scRNA-seq

DCs and monocytes play central roles in pathogen sensing, phagocytosis and antigen presentation, and are composed of a variety of specialized subtypes. However, their identities and interrelationships are not fully understood. Villani et al. isolated approximately 2,400 cells from healthy individuals and subjected them to scRNA-seq with follow-up profiling and functional and phenotypic characterization. Using the scRNA-seq of 2,400 cells, they identified six DCs and four monocyte subtypes in human blood. They revealed a new subset of DCs that shares properties with pDCs, thus redefining pDC; a new subdivision within the CD1c⁺ subset of DCs; the relationship between blastic plasmacytoid DC neoplasia cells and healthy DCs; and circulating progenitor of cDCs. The revised taxonomy will enable more accurate functional and developmental analysis and immune surveillance in health and disease.

Fig.2 Establishing a human blood monocyte and DC atlas. scRNA-seq and subsequent profiling and functional and phenotypic characterization of the isolated 2,400 cells led us to update the original cell classification to include six DCs, four monocyte subtypes and one conventional DC progenitor. (Villani, 2017)

Highlight Features

• The latest single-cell isolation and sequencing technologies
• Experienced staff to assist you with program design
• Fast turnaround time
• Accurate bioinformatics analysis
• Technical support to assist with data interpretation

With senior scientists and more than 10 years' CRO services, Creative Biolabs is committed to facilitating the success of your single cell programs leveraging our comprehensive and advanced single cell multiomics platforms. For more details, please feel free to contact us.

References

1. Villani, Alexandra-Chloé.; et al. Single-cell RNA-seq reveals new types of human blood dendritic cells, monocytes, and progenitors. Science. 2017, 356(6335):4573.
2. Gaëlle, Breton.; et al. Human dendritic cells (DCs) are derived from distinct circulating precursors that are precommitted to become CD1c+ or CD141+ DCs. Journal of Experimental Medicine. 2016, 213(13):2861-2870.